What is Kymograph used for?

What is Kymograph used for?

invention by Ludwig In 1847 he invented the kymograph, a cylindrical drum used to record muscular motion, changes in blood pressure, and other physiological phenomena.

What is a Kymograph in biology?

Kymographs are spacetime plots which display intensity values along a predefined path over time. Thus, in contrast to maximum projections, they can resolve time information.

Who invented the first Kymograph?

Carl Ludwig

What is the composition of fixing solution used for Kymograph paper?

This was carried out by carefully separating the smoked paper from the drum carrying the smoked paper in both hands and passing slowly moving the paper in a puddle of fixing solution consisting of alcoholic solution of shellac, colophony resin or other varnishing solution.

What is the composition of fixing solution?

The fixing solutions at present in use are: Cyanide of potassium, Hyposulphite of soda, and Sulphocyanide of ammonium.

Which paper is used for recording on the organ bath in laboratory?

Kymogram

What is magnification value in pharmacology?

• Magnification : = Distance from the fulcrum to the writing point Distance form the fulcrum to the tied tissue o For slow contracting muscles:- 10-15 times o For fast contracting muscles:-5-10 times. Step:4-Tissue selectionS.No Compound Tissue used1.

What is PSS in pharmacology?

1. System Preparation and Setup. Make 5 L of a physiological salt solution (PSS), which is the amount needed for a tissue bath contraction experiment that uses up to 50 ml tissue baths; see Table 2 for PSS recipe. Quantum sufficit PSS solution to 5 L.

Why is guinea pig ileum used in experiments?

A computer simulation program to teach the effects of drugs and electrical stimulation on the enteric nervous system. Its aim is to enable the exploration of the effects of drugs and electrical stimulation on the release of, and response to, neurotransmitters in the enteric nervous system. …

Which effect is produced by acetylcholine on the ileum of guinea pig?

The contractile effect of ACh on guinea pig ileum is mediated via muscarinic receptors, and is competitively and selectively blocked by atropine. Histamine induced contraction of guinea pig ileum is mediated by H1 receptors in which the latter effect is antagonized by mepyramine [12, 13].

How does ACh cause contraction of the ileum?

Abstract. Contraction of smooth muscle by acetylcholine is mediated by activation of muscarinic receptors of which M2 and M3 subtypes are present in longitudinal muscle of guinea pig intestine. These channels were studied by voltage-clamp of single cells from longitudinal muscle of rabbit small intestine.

Is Mepyramine a competitive antagonist?

The effects resulting from interaction with the histamine H2-receptors were revealed by treatment with mepyramine, a competitive antagonist of histamine at the H1-receptor.

What is the difference between competitive and noncompetitive antagonist?

A competitive antagonist binds to the same site as the agonist but does not activate it, thus blocks the agonist’s action. A non-competitive antagonist binds to an allosteric (non-agonist) site on the receptor to prevent activation of the receptor.

Is atropine a reversible competitive antagonist?

This occurs because atropine is a competitive, reversible antagonist of the muscarinic acetylcholine receptors (acetylcholine being the main neurotransmitter used by the parasympathetic nervous system). Atropine is a competitive antagonist of the muscarinic acetylcholine receptor types M1, M2, M3, M4 and M5.

Is atropine a non competitive antagonist?

Antagonism Histamine-atropine Atropine is a weak histamine antagonist, being about 1,000 times less active against histamine than against acetylcholine (Schild, 1947a). Nevertheless the antagonism between histamine and atropine is probably not unspecific but competitive.

Is atropine reversible or irreversible?

These inhibitory effects of atropine to agonists are generally believed to be reversible after washout, and there is no report about the irreversible inhibitory effect of atropine on the drug-induced contractions in smooth muscle organs.

Why Atropine is not used in motion sickness?

However, scopolamine has greater effects on the central nervous system (CNS) than atropine due to its ability to cross the blood–brain barrier. Scopolamine (Hyoscine) has anti-emetic activity and is, therefore, used to treat motion sickness. Antimuscarinics are also used as anti-parkinsonian drugs.

How do you know if an antagonist is competitive?

Antagonist: A drug that reduces the action of another drug.A competitive antagonist competes for the same binding site with an agonist, and their binding is mutually exclusive. A non-competitive antagonist can prevent the action of an agonist without any effect on the binding of the agonist to the receptor.

Is the antagonist good or bad?

The traditional definition of antagonist is a villain—a “bad guy” in the story, often working for evil purposes to destroy a heroic protagonist.

Is caffeine an agonist or antagonist?

Caffeine acts as an adenosine-receptor antagonist. This means that it binds to these same receptors, but without reducing neural activity. Fewer receptors are thus available to the natural “braking” action of adenosine, and neural activity therefore speeds up (see animation).

What is kymograph used for?

What is kymograph used for?

invention by Ludwig In 1847 he invented the kymograph, a cylindrical drum used to record muscular motion, changes in blood pressure, and other physiological phenomena. He also made significant contributions to the physiology of circulation and urine secretion.

What is a kymograph biology?

A kymograph is the two-dimensional representation of position versus time, taken directly from imaging data (Fig. 8.3B and D). In a kymograph, individual mitochondria appear as lines, and their average velocity is the slope of the line (De Vos & Sheetz, 2007; Miller & Sheetz, 2004).

What is a kymograph image?

Kymographs are graphical representations of spatial position over time, which are often used in biology to visualise the motion of fluorescent particles, molecules, vesicles, or organelles moving along a predictable path.

What is kymograph in pharmacology?

Kymograph, consisting of a revolving drum, bearing a record sheet (usually of smoked paper) on which a stylus moved up and down displaying the effects of the drugs on contractile tissues, was commonly used in experimental physiology and pharmacology laboratories in teaching institutions.

How many types of kymograph paper are there?

Five types of kymograph recorders or magnetic markers have been used.

What is use of frontal writing lever?

Frontal writing lever: The lever is designed in such a way that the writing point rotates freely about its axle. This helps in reducing the tension between the smoked paper and the recording tip. The contractions are recorded frontally as straight lines.

Who invented the first kymograph?

Carl Ludwig designed the kymograph, or wave writer, in 1847 (Figure 1). Its initial purpose was to improve Poiseuille’s manometer, which Ludwig used for the registration of blood pressure in experimental animals [11].

How does a Kymograph work?

The Kymograph consists of a revolving drum wrapped in paper and a stylus that moves back and forth recording any changes such as pressure or motion on that paper. The paper would have time intervals recorded on it which allowed someone to then record the data.

How do I download a ImageJ plugin?

How to install a plugin?

  1. I only have the the Java source file. Copy the *. java file to the ImageJ/Plugins folder or subfolder. Go to the menu entry Plugins>Compile and Run… and select the Java file you copied.
  2. I have the *. class file. Copy the *.
  3. I have the *. jar file. Copy the *.jar file to the ImageJ/Plugins folder.

What is the use of Sherrington Starling kymograph?

Kymograph consisting of a revolving drum, bearing a kymograph paper on which a stylus moves up and down displaying the effects of the drugs on contractile tissues, is commonly used in experimental physiology and pharmacology laboratories in teaching institutions.

What is the use of universal lever?

a machine formed by a combination of a lever with the wheel and axle, in such a manner as to convert the reciprocating motion of the lever into a continued rectilinear motion of some body to which the power is applied.

Who designed kymograph?

Carl Ludwig
Carl Ludwig designed the kymograph, or wave writer, in 1847 (Figure 1). Its initial purpose was to improve Poiseuille’s manometer, which Ludwig used for the registration of blood pressure in experimental animals [11].

Which is the correct definition of a kymograph?

Below: kymograph recording ICD10 = A kymograph (from Greek κῦμα, swell or wave + γραφή, writing; also called a kymographion) is a device that draws a graphical representation of spatial position over time in which a spatial axis represents time.

How is a manometer connected to a kymograph?

One limb of the manometer is connected to a stylus which writes on the kymograph. Below: kymograph recording ICD10 = A kymograph (from Greek κῦμα, swell or wave + γραφή, writing; also called a kymographion) is a device that draws a graphical representation of spatial position over time in which a spatial axis represents time.

What did Carl Ludwig use the kymograph for?

The kymograph was initially a mechanical and hydraulic device, invented by German physiologist Carl Ludwig in the 1840s, and found its first use as a means to monitor blood pressure. The blood pressure was conveyed by hydraulics and levers to move a stylus that scratched a white trace into soot-covered paper on the revolving drum.

How do you generate a kymograph in metamorph?

To generate a kymograph, first choose a sister kinetochore pair that is well separated from other kinetochores and any other background fluorescence. In MetaMorph, use the “Kymograph Function” to draw a line through the kinetochore pair of interest and create a cross-sectional view of the pair over time.

What is the medical definition of a kymograph?

kymograph – Medical Definition. n. An instrument for recording variations in pressure, as of the blood, or in tension, as of a muscle, by means of a pen or stylus that marks a rotating drum.

Who was the inventor of the kymograph drum?

invention by Ludwig. In physiology: Historical background In 1847 he invented the kymograph, a cylindrical drum used to record muscular motion, changes in blood pressure, and other physiological phenomena.

One limb of the manometer is connected to a stylus which writes on the kymograph. Below: kymograph recording ICD10 = A kymograph (from Greek κῦμα, swell or wave + γραφή, writing; also called a kymographion) is a device that draws a graphical representation of spatial position over time in which a spatial axis represents time.

How are writing levers used in the kymograph?

It is a standalone recording apparatus used alongside other apparatus such as organ bath. Writing levers are used to trace the recording from muscle contractions. Some of the commonly used writing levers are simple lever, frontal writing lever and starling heart lever to name a few.

Kymograph. (Science: physiology) An instrument for measuring, and recording graphically, the pressure of the blood in any of the blood vessels of a living animal.

What is kymograph in polygraph?

The kymograph was used as early as the 19th century for the simultaneous record- ing of data coming from the pneumograph and sphygmograph. The device thus constructed was known as a “polygraph”, i.e. a device for recording more than one function (Greek: poloi – multiple, graphos – to write).

Which lever is used for recording the contraction of heart?

Starling’s heart lever
Starling’s heart lever: It consists of a frame carrying a light lever arm with holes and notches supported by a fine adjustable spring attached to an adjustable hook. It is used to record the contractions of the heart.

German physiologist Carl Ludwig invented the kymograph in the 1840s and primarily used it to study changes in blood pressure as well as speech sounds.

What are the four phases of polygraph examination?

A polygraph examination may contain four (4) phases known as the Pre-test phase, Testing phase, Chart analysis phase and Post-test phase, any one of which constitutes a polygraph examination.

What is the use of Sherrington rotating drum?

Microteknik. This is an electrically driven recording drum or kymograph suitable for use by students for lab. Experiments. These kymographs are also used to measure atmospheric pressure, tuning fork vibrations and functioning of steam engines.

What are the uses of Sherrington rotating drum?

This is an electrically driven recording drum or kymograph suitable for use by students for lab. Experiments. These kymographs are also used to measure atmospheric pressure, tuning fork vibrations and functioning of steam engines.

What is the composition of fixing solution used for kymograph paper?

This was carried out by carefully separating the smoked paper from the drum carrying the smoked paper in both hands and passing slowly moving the paper in a puddle of fixing solution consisting of alcoholic solution of shellac, colophony resin or other varnishing solution.

What are the types of lever?

According to where the load and effort are located with respect to the fulcrum, there are three types or classes of lever:

  • First-Class Lever.
  • Second Class Lever.
  • Third Class Lever.

    What machines use a lever?

    Examples of levers in everyday life include teeter-totters, wheelbarrows, scissors, pliers, bottle openers, mops, brooms, shovels, nutcrackers and sports equipment like baseball bats, golf clubs and hockey sticks. Even your arm can act as a lever.

    What are the stages of polygraph?

    How accurate is the polygraph examination?

    There have been several reviews of polygraph accuracy. They suggest that polygraphs are accurate between 80% and 90% of the time. This means polygraphs are far from foolproof, but better than the average person’s ability to spot lies, which research suggests they can do around 55% of the time.

    What is the unit of Sherrington rotating drum machine?

    Carewell Sherrington Rotating Drum, Rs 5850 /unit Carewell Instrument | ID: 4211019648.

    Which paper is used for recording of activity on recording drum?

    A wax paper in place of smoked paper was suggested for use by Gerbrands and Volkmann. [2] One could use a simple glazed paper without smoking, but the ink writing pen device would be required for obtaining tracings.

    How do organ baths work?

    Introduction: The organ bath is a traditional experimental set-up that is commonly used to investigate the physiology and pharmacology of in vitro tissue preparations. The tissue reacts by contracting/relaxing and an isometric or isotonic transducer is used to measure force or displacement, respectively.

    What is the composition of fixing solution?

    Fixation is commonly achieved by treating the film or paper with a solution of thiosulfate salt. Popular salts are sodium thiosulfate—commonly called hypo—and ammonium thiosulfate—commonly used in modern rapid fixer formulae.